Dr Wendi's Health

How to Clone a Gene

How to Clone a Gene

This video describes the steps involved in cloning a gene, using bacteria and a human gene to make insulin. Also explained: restriction enzymes, vectors, and selectable markers.

Time Stamps

Definitions 0:10 

Gene cloning ingredients 2:16 

Restriction Enzymes 3:33 

Vectors 7:34 

Vector characteristics 8:36 

Typical Plasmid 10:08 

Other vectors 13:56 

Transformation vs transduction 14:38 

Cloning a gene 16:28 

Restriction map 16:51 

Cloning a gene continued 18:37 

Selectable markers 21:29 

Summary chart of medications made by bacteria 25:31 

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